NELFE Monoclonal Antibody-MB11292

NELFE Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11292-50, MB11292-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P18615

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 43 kDa; Observed MW: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NELFe in C6 lysates using NELFE antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NELFe antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: RD; RDP; RDBP; D6S45; NELF-E

Immunogen: Recombinant protein of human NELFe

Conjugate: Unconjugated

Modification: Unmodified