Product Description

Nephrin (H892) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS3462-50, BS3462-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Nephrin is a member of the immunoglobμlin family of cell adhesion molecμles that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selectiveμltrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1, 241 amino acid protein that contains a transmembrane domain, 8 Ig-like modμles, and 1 fibronectin III-like modμle. Nephrin is expressed in embryonic and adμlt kidneys and localizes to glomerμlar podocytes and the glomerμlar slit diaphragm. Nephrin stimμlates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Nephrin, Renal glomerμlus-specific cell adhesion receptor, NPHS1, NPHN

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 861-910 of Human Nephrin.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Nephrin (H892) polyclonal antibody detects endogenous levels of Nephrin protein.

Molecular Weight: ~ 135 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O60500

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only