Product Description

Nephrin (H892) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3462-50, BS3462-100

Sizes: 50µl, 100µl

Swiss-Prot: O60500

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Nephrin (H892) polyclonal antibody detects endogenous levels of Nephrin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Nephrin (H892) polyclonal antibody at 1:1000 dilution Lane 1: The muscle tissue lysate of Rat (47ug) Lane 2: HEK293T whole cell lysate (41ug) Lane 3: Jurkat whole cell lysate (41ug)
Lane 4: H9C2 whole cell lysate (60ug) , Immunohistochemistry (IHC) analyzes of Nephrin (H892) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 135 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1; NPHN

Immunogen: Synthetic peptide, corresponding to amino acids 861-910 of Human Nephrin.

Conjugate: Unconjugated

Modification: Unmodified