Nephrin Monoclonal Antibody-MB66728

Nephrin Monoclonal Antibody

Catalogue Numbers: MB66728-50, MB66728-100

Sizes: 50µl, 100µl

Product: Mouse IgM kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: O60500

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.12 and encodes a 1,241 amino acid protein that contains a transmembrane domain, eight Ig-like modules, and one fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Nephrin protein.

Extra Notes: Western blot analysis of Nephrin expression in HepG2 (A) whole cell lysates.

Bioworld Molecular Weight: ~ 180 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: NPHN; Nephrin; Renal glomerulus-specific cell adhesion receptor

Immunogen: Recombinant fusion protein of human Nephrin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified