Product Description

Nephrin Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66728-50, MB66728-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Nephrin is a member of the immunoglobμlin family of cell adhesion molecμles that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selectiveμltrafiltration. The human nephrin gene maps to chromosome 19q13.12 and encodes a 1, 241 amino acid protein that contains a transmembrane domain, eight Ig-like modμles, and one fibronectin III-like modμle. Nephrin is expressed in embryonic and adμlt kidneys and localizes to glomerμlar podocytes and the glomerμlar slit diaphragm. Nephrin stimμlates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with phorbol-12-myristate-13-acetate can upregμlate nephrin, suggesting that protein kinase C is part of an intracellμlar signalling system, which regμlates nephrin.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: NPHN, Nephrin, Renal glomerμlus-specific cell adhesion receptor

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human Nephrin. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of Nephrin protein.

Molecular Weight: ~ 180 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O60500

Product: Mouse IgM kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only