Product Description

Nephrocystin-5 (R467) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3319-50, BS3319-100

Sizes: 50µl, 100µl

Swiss-Prot: Q15051

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The nephrocystin proteins comprise a family of five enzymes that commonly interact with p130Cas, proline-rich tyrosine kinases, calmodulin and tensin, indicating that these proteins may participate in a common signaling pathway. Localized to the outer segments and primary cilia of photoreceptor cells, nephrocystin-5 is complexed with RPGR (retinitis pigmentosa GTPase regulator) and interacts directly with calmodulin. Nephrocystin-5 is thought to participate with RPGR in a pathway of ciliary function in the kidey and retina. Mutations in the gene encoding nephrocystin-5 are the primary cause of Senior-Loken syndrome 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Nephrocystin-5 (R467) polyclonal antibody detects endogenous levels of Nephrocystin-5 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Nephrocystin-5 (R467) pAb at 1:1000 dilution
Lane 1: The Kidney tissue lysate of Mouse (5ug)
Lane 2: The Ovary tissue lysate of Rat (40ug)
Lane 3: HCT116 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Nephrocystin-5 (R467) pAb in paraffin-embedded human colon carcinoma tissue.

Bioworld Molecular Weight: ~ 69 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: IQ calmodulin-binding motif-containing protein 1; Nephrocystin-5; p53 and DNA damage-regulated IQ motif protein; PIQ; IQCB1; KIAA0036; NPHP5; OK/SW-cl.85

Immunogen: Synthetic peptide, corresponding to amino acids 432-486 of Human Nephrocystin-5.

Conjugate: Unconjugated

Modification: Unmodified