Nestin Polyclonal Antibody-BS65858

Nestin Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65858-50, BS65858-100

Swiss-Prot: Q6P5H2

Host: Rabbit

Nestin Polyclonal Antibody-BS65858

Storage and Stability: Store at 4°C short term. Aliquot and store at -31°C long term. Avoid freeze-thaw cycles.

Reactivity: Mouse, Rat,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Purification and Purity: affinity purified by Protein A

Specificity: Nestin Polyclonal Antibody detects endogenous levels of Nestin protein.

Bioworld Molecular Weight: ~209kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (rat brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nestin) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Mouse brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nestin) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C

Alternative Name: ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_MOUSE; Nestin.

Immunogen: KLH conjugated synthetic peptide derived from mouse Nestin:1231-1330/1864

Modification: Unmodified

Conjugate: Unconjugated