Product Description

NET (Phospho-S357) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64526-50, BS64526-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P41970

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: Numerous cellular functions, such as proliferation, differentiation, apoptosis, vesicular trafficking, nuclear transport and cytoskeletal organization, are controlled by GTPases. It has become increasingly clear that GTPases act in cascades in which their activities are linked by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). Researchers looking for new epithelial cell-specific oncogenes, using a highly efficient cDNA expression cloning system, have isolated the Ost oncogene from rat osteosarcoma cells. The Ost proto-oncogene protein contains DH and PH domains, catalyzes guanine nucleotide exchange on Rho A and Cdc42, and interacts specifically with the GTP-bound form of Rac1. The related NET1 protein also contains a DH domain and is ubiquitously expressed in a variety of tissues. Overexpression of NET1 in NIH/3T3 cells results in altered growth properties and tumorigenesis when injected into nude mice.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NET (Phospho-S357) polyclonal antibody detects endogenous levels of NET protein only when phosphorylated at Ser357.

Bioworld Molecular Weight: ~ 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NET (Phospho-S357) polyclonal antibody at 1:500 dilution
Lane A: Zebrafish

Alternative Name: ETS domain-containing protein Elk-3; ETS-related protein ERP; ETS-related protein NET; Serum response factor accessory protein 2; SAP-2; SRF accessory protein 2; ELK3; NET; SAP2

Immunogen: Synthetic phosphopeptide derived from human NET around the phosphorylation site of Serine 357.

Conjugate: Unconjugated

Modification: Phosphorylation