NeuN Rabbit Monoclonal Antibody-BS9837M

NeuN Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9837M-50, BS9837M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: A6NFN3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:200-1:500
FC: 1:50-1:100

Background: Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of NeuN and does not cross-react with related proteins.

Bioworld Molecular Weight: Predicted band size: 34KDa
Observed band size: 46, 48KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NeuN Rabbit mAb at 1:1000 dilution
Lane 1: The brain tissue lysate of Human
( NeuN is expressed as two alternate transcripts
with apparent SDS-PAGE molecular weight 46 and 48kDa), Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin., ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS

Alternative Name: RNA binding protein fox-1 homolog 3; Fox-1 homolog C; RBFOX3; NeuN; Fox-3; Fox3; Neuronal Marker

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified