Neutrophil Elastase (ELANE) Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A13015-20, A13015-100
Citations, Manuals and MSDS Available upon request.
Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, IF-P, IHC-P, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 29kDa
Observed Molecular Weight: 35kDa
Immunogen: Synthetic peptide
Species: Human
GeneID: 1991
SWISS: P08246
Alternate Names: GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; Neutrophil Elastase (ELANE)
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IF/ICC, 1:50 - 1:200 IF-P, 1:50 - 1:200 IHC-P, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Signal Transduction, Cell Biology Developmental Biology, Cytoskeleton, Extracellular Matrix.
NCBI Alias: ELANE
Research Use Only