Product Description

Neutrophil Elastase Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90933-50, BS90933-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08246(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000
ICC:1:50
IHC:1:50-1:200
FC:1:10-1:50

Background: Neutrophil Elastase (NE) is a serine protease that is expressed in bone marrow precursor cells, stored in peripheral blood granulocytes, and implicated in the progression of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome and cystic fibrosis. In neutrophils, Neutrophil Elastase contributes largely to the proteolysis of phagocytosed proteins, the migration of neutrophils and the remodeling of tissues following injury. Neutrophil Elastase, which is also designated medullasin, is secreted into the extracellular matrix, where it is then capable of destroying connective tissue proteins, including elastin, proteoglycans and Type IV Collagens. Neutrophil Elastase also mediates proteolysis by cleaving proteins that are associated with the complement system, such as antithrombin and Fibrinogen. Additionally, Neutrophil Elastase functions as a potent platelet agonist, where it potentiates the aggregation, secretion and mobilization of calcium in response to cathepsin G binding to platelet surface receptors.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Neutrophil Elastase polyclonal antibody detects endogenous levels of Neutrophil Elastase protein.

Bioworld Molecular Weight: 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Neutrophil Elastase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HLE, ELANE, Bone marrow serine protease, ELA2, ELANE, Elastase 2, Elastase 2 neutrophil, Elastase neutrophil expressed, Elastase-2, ELNE, GE, Granulocyte derived elastase, HLE, HNE, Human leukocyte elastase, Leukocyte elastase, Medullasin, NE, Neutrophil elastase, PMN E, PMN elastase, Polymorphonuclear elastase, SCN1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified