Product Description

NF-κB p100 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Catalogue Numbers: AP0219-50, AP0219-100

Sizes: 50µl, 100µl

Swiss-Prot: Q00653

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IF

Background: Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NF-κB p100 polyclonal antibody detects endogenous levels of NF-κB p100 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NF-κB p100 polyclonal antibody at 1:1000 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: The Kidney tissue lysate of Rat (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: BV2 whole cell lysate (40ug), Immunofluorescence analysis of A549 cells using NF-κB p100 antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 120 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10; Nuclear factor NF-kappa-B p52 subunit

Immunogen: Synthetic peptide, corresponding to Human NF-Κb p100

Conjugate: Unconjugated

Modification: Unmodified