Product Description

NF-KB p65 (3D2) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3198-50, MB3198-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q04206

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 60 kDa; Observed MW: 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NF-KB p65 in T47D, 3T3, COS7, C6 and Hela lysates using NF-KB p65 antibody., Immunofluorescence analysis of NF-KB p65 in Hela using NF-KB p65 antibody, and DAPI., Immunoprecipitation analysis of NF-KB p65 in Hela lysates using NFκB p65 antibody., Immunohistochemistry analysis of paraffin-embedded rat Brain Tissue using NF-KB p65 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

Immunogen: Recombinant Protein of Transcription factor p65

Conjugate: Unconjugated

Modification: Unmodified