Product Description

NF-YC (W85) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2428-50, BS2428-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13952

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold “handshake” motif in a manner analogous to the histone proteins, H2B and H2A, respectively.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NF-YC (W85) polyclonal antibody detects endogenous levels of NF-YC protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NF-YC (W85) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of NF-YC (W85) pAb in paraffin-embedded liver cancer tissue at 1:100.

Bioworld Molecular Weight: ~ 50 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Nuclear transcription factor Y subunit gamma; CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; NF-YC; Transactivator HSM-1/2; NFYC

Immunogen: Synthetic peptide, corresponding to amino acids 50-106 of Human NF-YC.

Conjugate: Unconjugated

Modification: Unmodified