NFATc4 (phospho Ser168/S170) rabbit pAb - ES6370

NFATc4 (phospho Ser168/S170) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES6370-50, ES6370-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],

Alternate Name: NFATC4; NFAT3; Nuclear factor of activated T-cells; cytoplasmic 4; NF-ATc4; NFATc4; T-cell transcription factor NFAT3; NF-AT3

Source: Rabbit

Applications: WB; IHC; IF; ELISA

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Reactivity: Human; Mouse

Immunogen: The antiserum was produced against synthesized peptide derived from human NFAT3 around the phosphorylation site of Ser168 and Ser170. AA range:136-185

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 140kD

Human Gene ID: 4776

Human SWISS Prot NO: Q14934

Subcellular Location: Cytoplasm, cytosol. Nucleus. When hyperphosphorylated, localizes in the cytosol. When intracellular Ca (2+) levels increase, dephosphorylation by calcineurin/PPP3CA leads to translocation into the nucleus (PubMed:11997522, PubMed:18347059). MAPK7/ERK5 and MTOR regulate NFATC4 nuclear export through phosphorylation at Ser-168 and Ser-170 (PubMed:18347059).

Research Use Only