NFIX Polyclonal Antibody-BS71797

NFIX Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71797-50, BS71797-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14938

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:1000

Background: The protein encoded by this gene is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. The encoded protein can also stimulate adenovirus replication in vitro. Three transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using NFIX antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

Bioworld Molecular Weight: 50kDa

Specificity: Polyclonal Antibodies

Alternative Name: NFIX; CTF; MRSHSS; NF-I/X; NF1-X; NF1A; SOTOS2

Immunogen: Recombinant fusion protein of human NFIX(NP_002492.2).

Conjugate: Unconjugated

Modification: Unmodified