Product Description

NFkB-p100 (phospho-S865) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4129-50, BS4129-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimμlate the HIV enhancer in transiently transfected Jurkat cells.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light, polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10, NFKB2, LYT10

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human NFkB-p100 around the phosphorylation site of Serine 865.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-NFkB-p100 (S865) polyclonal antibody Polyclonal Antibody detects endogenous levels of NFkB-p100 protein only when phosphorylated at Ser865.

Molecular Weight: ~ 100, 120 kDa

Pathway: NF-kB Signaling, STING Signaling, Inhibition of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q00653

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only