Product Description

NFkB-p100 (phospho-S869) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS4130-50, BS4130-100

Sizes: 50µl, 100µl

Swiss-Prot: Q00653

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-NFkB-p100 (S869) polyclonal antibody pAb detects endogenous levels of NFkB-p100 protein only when phosphorylated at Ser869.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of p-NFkB-p100 (S869) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 100, 120 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light; polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10; NFKB2; LYT10

Immunogen: Synthetic phosphopeptide derived from human NFkB-p100 around the phosphorylation site of Serine 869.

Conjugate: Unconjugated

Modification: Phosphorylation