Product Description

NFkB p100 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS90938-50, BS90938-100

Swiss-Prot: Q00653

Host: Rabbit

NFkB p100 Polyclonal Antibody-BS90938

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB:1:5000~1:10000
IF: 1:50~1:200

Background: NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NFkB p100 polyclonal antibody detects endogenous levels of NFkB p100 protein.

Bioworld Molecular Weight: ~ 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NFkB p100 pAb at 1:5000 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: U-87MG whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug), Immunofluorescence analysis of A549 cells using NFkB p100 antibody at dilution of 1:50.

Alternative Name: Lyt10; NFKB2; LYT10; CVID10; DNA binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; NF kB2; NFKB p52/p100 subunit; Nuclear factor Kappa B subunit 2; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100); Nuclear factor of kappa light polypeptide gene enhancer in B cells 2; Oncogene Lyt 10; p100; Transcription factor NFKB2

Immunogen: Recombinant protein.

Modification: Unmodified

Conjugate: Unconjugated