NFS1 Polyclonal Antibody-BS8339

NFS1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8339-50, BS8339-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y697

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: NFS1 (nitrogen fixation 1), also known as NIFS or IscS (cysteine desulfurase), is a member of the class V pyridoxal-phosphate-dependent aminotransferase family. It localizes to the cytoplasm or mitochondrion depending on which form is generated based on cytosolic pH. Highest expression levels of NFS1 are found in heart and skeletal muscle. Lower levels of expression are also found in liver, brain and pancreas. NFS1 is responsible for catalyzing the removal of sulfur from cysteine to form alanine, thereby supplying the inorganic sulfur for iron-sulfur (Fe-S) clusters. Fe-S clusters function as essential cofactors in a wide variety of events, including facilitation of electron transfer processes in oxidative phosphorylation, catalysis of enzymatic reactions in aconitase and dehydratases and maintenance of structural integrity in the DNA repair enzyme endonuclease III.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NFS1 polyclonal antibody detects endogenous levels of NFS1 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of NFS1 polyclonal antibody

Alternative Name: Cysteine desulfurase, mitochondrial; NFS1; NIFS; HUSSY-08

Immunogen: Recombinant full length Human NFS1.

Conjugate: Unconjugated

Modification: Unmodified