Product Description

NG2/CSPG4 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS80367-50, BS80367-100

Swiss-Prot: Q6UVK1

Host: Rabbit

NG2/CSPG4 Polyclonal Antibody-BS80367

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded Mouse brain using NG2/CSPG4 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of A-375 cells using [KO Validated] NG2/CSPG4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2; chondroitin sulfate proteoglycan 4

Immunogen: Recombinant fusion protein of human NG2/CSPG4(NP_001888.2).

Modification: Unmodified

Conjugate: Unconjugated