Product Description

NG2/CSPG4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80386-50, BS80386-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8VHY0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:200 - 1:2000

Background: Enables coreceptor activity. Acts upstream of or within several processes, including platelet-derived growth factor receptor signaling pathway; positive regulation of MAPK cascade; and ruffle assembly. Located in plasma membrane and ruffle. Is expressed in several structures, including brain; jaw; respiratory system; retina; and skeleton. Human ortholog(s) of this gene implicated in relapsing-remitting multiple sclerosis. Orthologous to human CSPG4 (chondroitin sulfate proteoglycan 4).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 270-300KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using NG2/CSPG4 antibody at 1:1639 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Alternative Name: AN2; NG2; Cspg4a; 4732461B14Rik

Immunogen: Recombinant fusion protein of human NG2/CSPG4NG2/CSPG4.

Conjugate: Unconjugated

Modification: Unmodified