Product Description

NGF (L82) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3564-50, BS3564-100

Sizes: 50µl, 100µl

Swiss-Prot: P01138

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NGF (L82) polyclonal antibody detects endogenous levels of Pro-NGF protein, this antibody does not cross-react with Mature-NGF.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NGF (L82) pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate (40ug)
Lane 2: Panc1 whole cell lysate (40ug)
Lane 3: CT26 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of NGF (L82) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 34 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Beta-nerve growth factor; Beta-NGF; NGFB

Immunogen: Synthetic peptide, corresponding to amino acids 51-100 of Human NGF.

Conjugate: Unconjugated

Modification: Unmodified