NGF Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90942-50, BS90942-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01138(Human) P01139(Mouse) P25427(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, zebrafish
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: NGF polyclonal antibody detects endogenous levels of NGF protein.
Bioworld Molecular Weight: 32 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of NGF on Hela cell lysates using anti-NGF antibody at 1/1,000 dilution., ICC staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Beta-NGF, NGFB, Beta nerve growth factor, Beta NGF, Beta-nerve growth factor, Beta-NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor, Nerve growth factor beta, Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGF, NGF, NGFB, NID67,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified