Product Description

NGF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90943-50, BS90943-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01138(Human) P01139(Mouse) P25427(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200

Background: Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: NGF polyclonal antibody detects endogenous levels of NGF protein.

Bioworld Molecular Weight: 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NGF on recombinant protein lysate using anti-NGF antibody at 1/500 dilution., ICC staining NGF in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Beta-NGF, NGF, NGFB, Beta nerve growth factor, Beta NGF, Beta-nerve growth factor, Beta-NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor, Nerve growth factor beta, Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGF, NGF, NGFB, NID67,

Immunogen: Synthetic peptide within Human NGF aa 100-150.

Conjugate: Unconjugated

Modification: Unmodified