Product Description

NHE-7 (D566) Polyclonal Antibody

Catalogue Numbers: BS3400-50, BS3400-100

Sizes: 50µl, 100µl

Alternative Name: Sodium/hydrogen exchanger 7; Na(+)/H(+) exchanger 7; NHE-7; Solute carrier family 9 member 7; SLC9A7; NHE7

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96T83

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. NHE7 (human 725 aa, chromosome Xp11.4) is ubiquitously expressed, and predominantly localizes to the trans-golgi network. NHE7 mediates the influx of Na+ or K+ in exchange for H+. It is ~70% related to NHE6 but relatively less (~25%) homologous with other NHEs.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NHE-7 (D566) polyclonal antibody detects endogenous levels of NHE-7 protein.

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NHE-7 (D566) pAb at 1:500 dilution
Lane 1: CT26 whole cell lysate(40ug)
Lane 2: PC12 whole cell lysate(40ug)
Lane 3: U-87MG whole cell lysate(40ug)
Lane 4: HCT116 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 530-582 of Human NHE-7.

Conjugate: Unconjugated

Modification: Unmodified