Product Description

Nibrin (phospho-S343) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4153-50, BS4153-100

Swiss-Prot: O60934

Host: Rabbit

Nibrin (phospho-S343) Polyclonal Antibody-BS4153

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-p95/NBS1 (S343) polyclonal antibody detects endogenous levels of p95/NBS1 protein when phosphorylated at Ser343.

Bioworld Molecular Weight: ~ 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-Nibrin (S343) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: HEK293T treated with UV for 5 minutes then repair for 1 hours whole cell lysate (40ug)
Lane 3: HEK293T treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-p95/NBS1 (S343) pAb in paraffin-embedded human tonsil tissue.

Alternative Name: Nijmegen breakage syndrome protein 1; Cell cycle regulatory protein p95; NBS1/P95; NBS; NBS1; P95

Immunogen: Synthetic phosphopeptide derived from human Nibrin around the phosphorylation site of Serine 343.

Modification: Phosphorylation

Conjugate: Unconjugated