Product Description

NIFK (D229) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1679-50, BS1679-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adμlt tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: MKI67 FHA domain-interacting nucleolar phosphoprotein, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67, hNIFK, NIFK, MKI67IP, NOPP34

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 196-245 of Human NIFK.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NIFK (D229) polyclonal antibody detects endogenous levels of NIFK protein.

Molecular Weight: ~ 34 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9BYG3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only