Product Description

NIPA (S350) Polyclonal Antibody

Catalogue Numbers: BS1847-50, BS1847-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q86WB0

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: Entry into mitosis is essentially driven by cyclin B1 which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear Interaction Partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NIPA (S350) polyclonal antibody detects endogenous levels of NIPA protein.

Extra Notes: Western blot (WB) analysis of NIPA (S350) pAb at 1:500 dilution
Lane1:PC3 whole cell lysate(20ug)
Lane2:K562 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(10ug)
Lane4:AML-12 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 60-65 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1; ZC3HC1; NIPA; HSPC216

Immunogen: Synthetic peptide, corresponding to amino acids 318-372 of Human NIPA.

Conjugate: Unconjugated

Modification: Unmodified