Product Description

NIPP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8541-50, BS8541-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12972

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: NIPP1 (nuclear inhibitor of protein phosphatase 1) is a putative transcription regulator that may be involved in pre-mRNA splicing and cell proliferation. NIPP1 contains a nuclear signaling region named FHA (fork-head associated) domain. The FHA domain has been associated with protein kinases and transcription factors. The NIPP1 locus encodes for three different isoforms termed α, β and γ due to alternative splicing events. The isoforms exhibit RNA binding activity and also act as phophatase inhibitors. The γ isoform is believed to be a magnesium dependent endoribonuclease that is responsible for cleaving RNA strands. It is mainly found in B cells and T lymphocytes. The α and β isoforms are localized in the brain and kidney. Inactivation of NIPP1 is accomplished by the phosphorylation of Ser 199 or Ser 204. NIPP1 interacts with proteins CDc5L, SAP 155, MELK and EED.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NIPP1 polyclonal antibody detects endogenous levels of NIPP1 protein.

Bioworld Molecular Weight: ~ 38kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of various cell lines, using PPP1R8 antibody.

Alternative Name: Nuclear inhibitor of protein phosphatase 1; NIPP-1; Protein phosphatase 1 regulatory inhibitor subunit 8; Activator of RNA decay; ARD-1; PPP1R8; ARD1

Immunogen: Recombinant full length Human NIPP1.

Conjugate: Unconjugated

Modification: Unmodified