Product Description

NLE1 (Y333) Polyclonal Antibody

Catalogue Numbers: BS3353-50, BS3353-100

Sizes: 50µl, 100µl

Alternative Name: Notchless protein homolog 1; NLE1; HUSSY-07

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NVX2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway.In plants, NLE1 is crucial for normal cellular growth and development. Underexpression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NLE1 (Y333) polyclonal antibody detects endogenous levels of NLE1 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NLE1 (Y333) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate(20ug)
Lane 2: K562 whole cell lysate(20ug)
Lane 3: HCT116 whole cell lysate(20ug)
Lane 4: PC12 whole cell lysate(40ug)
Lane 5: CT26 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 302-350 of Human NLE1.

Conjugate: Unconjugated

Modification: Unmodified