Product Description

NLK Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS7516-50, BS7516-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellμlar-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regμlated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both Thr-183 and Tyr-185 is required for fμll enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (5, 6). Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene. Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases. Nlk is a nuclear protein with the ability to autophosphorylate.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: LAK1, Nemo like kinase, Nemo-like kinase, Nlk, NLK_HUMAN, Protein LAK1, Serine/threonine protein kinase NLK, Serine/threonine-protein kinase NLK,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length Human NLK.

Dilution: WB: 1:500~1:2000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NLK polyclonal antibody detects endogenous levels of NLK protein.

Molecular Weight: ~ 68 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UBE8

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only