NLK Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS7516-50, BS7516-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UBE8
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both Thr-183 and Tyr-185 is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (5,6). Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene. Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases. Nlk is a nuclear protein with the ability to autophosphorylate.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: NLK polyclonal antibody detects endogenous levels of NLK protein.
Bioworld Molecular Weight: ~ 68 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western Blot analysis of NLK polyclonal antibody
Alternative Name: LAK1;
Nemo like kinase;
Nemo-like kinase;
Nlk;
NLK_HUMAN;
Protein LAK1;
Serine/threonine protein kinase NLK;
Serine/threonine-protein kinase NLK;
Immunogen: Recombinant full length Human NLK.
Conjugate: Unconjugated
Modification: Unmodified