Product Description

NLRP3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS66103-50, BS66103-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96P20

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF, IHC

All Applications: WB: 1:1000~1:3000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NLRP3 polyclonal antibody detects endogenous levels of NLRP3 protein.

Bioworld Molecular Weight: ~ 105 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using NLRP3 antibody at 1:500 dilution., Immunohistochemistry of paraffin-embedded rat lung using NLRP3 antibody at dilution of 1:100 (40x lens)., Immunohistochemistry of paraffin-embedded human appendix using NLRP3 antibody at dilution of 1:100 (40x lens)., Immunofluorescence analysis of Raji cells using NLRP3 Rabbit pAb at dilution of 1:150 (40x lens).

Alternative Name: NLRP3; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human NLRP3 (NP_001120934.1).

Conjugate: Unconjugated

Modification: Unmodified