Product Description

NLRP3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90948-50, BS90948-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q96P20

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ELISA IHC IF

All Applications: WB: 1:500-1:2000
ELISA: 1:5000-1:10000
IHC: 1:100-1:500
IF: 1:100-1:500

Background: Cryopyrin interacts selectively with apoptosis associated specklike protein containing a CARD domain (ASC). This complex may function as an upstream activator of NFkB signaling and caspase-1 activation. The complex also inhibits TNFa induced activation and nuclear translocation of RelA/NFkB p65. Mutations in Cryopyrin and Pyrin proteins are responsible for several autoinflammatory disorders in humans, including familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NLRP3 polyclonal antibody detects endogenous levels of NLRP3 protein.

Bioworld Molecular Weight: ~ 114 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
Lung (Rat) Lysate at 40 ug
Colon carcinoma (Human) Lysate at 40 ug
Primary: Anti-NALP3 at 1/300 dilution, Tissue/cell: Rat tracheal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Cryopyrin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.

Alternative Name: LRR and PYD domains-containing protein 3; AGTAVPRL; AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLRP3; PYPAF 1; PYPAF1 antibody PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1

Immunogen: KLH conjugated synthetic peptide derived from human Cryopyrin: 551-630/1036

Conjugate: Unconjugated

Modification: Unmodified