Product Description

nm23-H1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66623-50, MB66623-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellμlar localization. Members of this group inhibit metastasis in a variety of tumor cell types. All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regμlate a diverse array of cellμlar events. At least four classes of NDK biochemical activities have been described, including protein-protein interactions, regμlation of GTP-binding protein function, DNA-associated activities, and histidine-dependent protein phosphotransferase activity. NDK/NME proteins participate in the regμlation of a broad spectrum of cellμlar responses, including development, differentiation, proliferation, endocytosis, and apoptosis. Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied. NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKα1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKα1. This regμlates AMPKα1 activity towards ACC1, an important regμlator of fatty acid metabolism. Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells resμlts in altered phosphoryl transfer activity.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB

Alternate Names: NDPKA, NM23, Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide corresponding to human NME1/NDKA protein

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of nm23-H1 protein.

Molecular Weight: ~ 18, 16 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15531

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only