Product Description

Nmi Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67088-50, MB67088-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Acts as a signaling pathway regμlator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a mμltitude of signals for development and homeostasis. Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resμlting in increased STAT1- and STAT5-dependent transcription. In response to interferon IFN-alpha, associates in a complex with signaling pathway regμlator IFI35 to regμlate immune response, the complex formation prevents proteasome-mediated degradation of IFI35. In complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21. In complex with IFI35, negatively regμlates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65/RELA, resμlting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries . Negatively regμlates virus-triggered type I interferon/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regμlator of type I IFN, thereby interfering with cellμlar antiviral responses (By similarity). Beside its role as an intracellμlar signaling pathway regμlator, also functions extracellμlarly as damage-associated molecμlar patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellμlar space during cell injury or pathogen invasion. Macrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC, FC

Alternate Names: N-myc-interactor, Nmi, N-myc and STAT interactor

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human Nmi. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of Nmi protein.

Molecular Weight: ~ 40 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13287

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only