NNF1R Polyclonal Antibody-BS65456

NNF1R Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65456-50, BS65456-100

Swiss-Prot: Q6P1K2

Host: Rabbit

NNF1R Polyclonal Antibody-BS65456

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: NNF1R, also called PMF1, is part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. It is required for chromosome congression and for correct operation of the spindle checkpoint. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.

Purification and Purity: affinity purified by Protein A

Specificity: NNF1R Polyclonal Antibody detects endogenous levels of NNF1R protein.

Bioworld Molecular Weight: 34 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (rat heart)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (rat kidney)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Alternative Name: PMF-1; Pmf1; PMF1_HUMAN; NNF1R; Polyamine modulated factor 1; Polyamine-modulated factor 1.

Immunogen: KLH conjugated synthetic peptide derived from human NNF1R/PMF1:101-200/300

Modification: Unmodified

Conjugate: Unconjugated