Product Description

NNMT Polyclonal Antibody

Catalogue Numbers: BS8344-50, BS8344-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40261

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Nicotinamide N-methyltransferase (NNMT) catalyzes the N-methylation of nicotin-amide and other pyridines. NNMT activity in the human liver has a bimodal frequency distribution, indicating that its enzyme activity may be modulated through a genetic polymorphism, which could have functional implications for individual differences in drug and xenobiotic toxicity. The gene that encodes human NNMT is approximately 16.5 kb in length, consists of three exons and two introns and maps to 11q23.2. NNMT isolated from the human liver was determined to be 969 nucleotides in length, with a 792 nucleotide open reading frame that encodes a 264 amino acid protein. The NNMT gene is presumed to be a significant genetic determinant of plasma homocysteine levels in Spanish families, since it encodes an enzyme involved in homocysteine synthesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NNMT polyclonal antibody detects endogenous levels of NNMT protein.

Extra Notes: Western blot (WB) analysis of NNMT pAb at 1:1000 dilution
Lane1:The liver tissue lysate of Mouse(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:SK-OVCAR3 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Nicotinamide N-methyltransferase; NNMT

Immunogen: Recombinant full length Human NNMT.

Conjugate: Unconjugated

Modification: Unmodified