Product Description

NOC3L (F447) Polyclonal Antibody

Catalogue Numbers: BS3358-50, BS3358-100

Sizes: 50µl, 100µl

Alternative Name: Nucleolar complex protein 3 homolog; NOC3 protein homolog; Factor for adipocyte differentiation 24; NOC3-like protein; Nucleolar complex-associated protein 3-like protein; NOC3L; AD24; C10orf117; FAD24

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WTT2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: GADD 153, a growth arrest and DNA damage-inducible gene, encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10 or C/EBP zeta). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase during cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from “classical” C/EBP binding sites, recognizing instead unique “nonclassical” sites. Thus, GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells. GADD 153 belongs to the CBF/MAK21 family, which also includes NOC2L, NOC3L and NOC4L. NOC3L, also designated factor for adipocyte differentiation 24 or Fad24, promotes adipogenesis by controlling DNA replication during the early stages of mitotic clonal expansion (MCE).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NOC3L (F447) polyclonal antibody detects endogenous levels of NOC3L protein.

Bioworld Molecular Weight: ~ 93 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NOC3L (F447) pAb at 1:1000 dilution
Lane 1: HCT116 whole cell lysate(20ug)
Lane 2: HepG2 whole cell lysate(20ug)
Lane 3: AML-12 whole cell lysate(20ug)
Lane 4: H9C2 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 417-436 of Human NOC3L.

Conjugate: Unconjugated

Modification: Unmodified