Noggin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90954-50, BS90954-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13253(Human) Q62809(Rat)
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Genetic differentiation of the vertebrate somite necessitates a balance of inductive signals and antagonists. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGFβ) superfamily of signaling proteins, such as bone morphogenetic proteins-2, 4, 7 (BMP2, BMP4, BMP7). Inhibition of BMP signaling by axially secreted Noggin mediates normal vertebrate skeletogenesis and patterning of the neural tube and somite. Spatially, Noggin may effectively antagonize BMP activity by efficiently diffusing through extracellular matrices, thereby creating morpho-genic gradients. Mice embryos that are homozygous null for Noggin, a lethal genotype, display stubby, continuous limbs with lack of joints in the paws and an array of other developmental defects.
Purification and Purity: Protein affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Noggin polyclonal antibody detects endogenous levels of Noggin protein.
Bioworld Molecular Weight: 26 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution., ICC staining Noggin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: NOG, Nog, NOGG, Noggin, SYM 1, SYM1, Symphalangism 1 (proximal), Synostoses (multiple) syndrome 1, SYNS 1, SYNS1,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified