Nogo A (E499) Polyclonal Antibody-BS2888

Nogo A (E499) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2888-50, BS2888-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9NQC3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: CNS white matter is selectively inhibitory for axonal out-growth. Nogo (also designated NI250 and Reticulon 4-A) is an oligodendrocyte-specific member of the Reticulon family and is a component of CNS white matter that inhibits axon outgrowth, induces collapse of growth cones of chick dorsal root ganglion cells, and inhibits the spreading of 3T3 fibroblasts. Other members of the Reticulon protein family do not inhibit axon xtension and are thought to have a role in ER function. Nogo is expressed by oligodendrocytes but not by Schwann cells, and associates primarily with the endoplasmic reticulum. Nogo exists in three different splice forms, Nogo A, Nogo B and Nogo C.Nogo A and Nogo C have a molecular mass of 200-250 kDa and 22 kDa,
respectively.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Nogo A (E499) polyclonal antibody detects endogenous levels of Nogo A protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Nogo A pAb at 1:500 dilution
Lane 1: The Brain tissue lysate of Mouse (40ug)
Lane 2: The Brain tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Nogo A (E499) pAb in paraffin-embedded human skeletal muscle tissue.

Bioworld Molecular Weight: ~ 130 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Reticulon-4; Reticulon 4; Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5; RTN4; KIAA0886; NOGO; My043; SP1507

Immunogen: Synthetic peptide, corresponding to amino acids 467-517 of Human Nogo A.

Conjugate: Unconjugated

Modification: Unmodified