Product Description

Nogo B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90955-50, BS90955-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NQC3(Human) Q99P72(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, IP, FC

All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Nogo B polyclonal antibody detects endogenous levels of Nogo B protein.

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution., ICC staining Nogo in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.