NOLC1 Monoclonal Antibody-MB11317

NOLC1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11317-50, MB11317-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q14978

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 74 kDa; Observed MW: 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NOLC1 in Jurkat lysates using NOLC1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NOLC1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunocytochemistry analysis of NOLC1 in Hela using NOLC1 antibody, and DAPI

Alternative Name: P130; NOPP130; NOPP140; NS5ATP13

Immunogen: A synthetic peptide of human NOLC1

Conjugate: Unconjugated

Modification: Unmodified