Product Description

NOP2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77904-50, BS77904-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P46087

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Involved in ribosomal large subunit assembly. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5 position of cytosine 4447 in 28S rRNA (Probable. May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using NOP2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 110KDa

Alternative Name: NOP2; NOL1; NOP120; NSUN1; p120

Immunogen: Recombinant fusion protein of human NOP2(NP_001245237.1).

Conjugate: Unconjugated

Modification: Unmodified