Product Description

NOS3 (G488) Polyclonal Antibody

Catalogue Numbers: BS3625-50, BS3625-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29474

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NOS3 (G488) polyclonal antibody detects endogenous levels of NOS3 protein.

Extra Notes: Western blot (WB) analysis of NOS3 pAb at 1:500 dilution
Lane1:THP-1 whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:AML-12 whole cell lysate(40ug)
Lane5:PC12 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 115 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3

Immunogen: Synthetic peptide, corresponding to amino acids 455-500 of Human NOS3.

Conjugate: Unconjugated

Modification: Unmodified