Product Description

NOX5 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67242-50, BS67242-100

Sizes: 50µl, 100µl

Swiss-Prot: Q96PH1

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

Background: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 203% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of NOX5 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NOX5 expression in rat kidney (A) whole cell lysates., Immunohistochemical analysis of NOX5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 78 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: NADPH oxidase 5

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NOX5. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified