Product Description

NPM (K230) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1804-50, BS1804-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06748

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of NPM (K230) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 38 kDa

Specificity: NPM (K230) polyclonal antibody detects endogenous levels of NPM protein.

Alternative Name: Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM

Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human NPM1.

Conjugate: Unconjugated

Modification: Unmodified