NQO1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90961-50, BS90961-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P15559(Human) Q64669(Mouse) P05982(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC FC
All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100
Background: NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: NQO1 polyclonal antibody detects endogenous levels of NQO1 protein.
Bioworld Molecular Weight: 31 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Siha
Lane 2: A549, Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
Alternative Name: DTD, QR1, NQO1, DIA4, NMOR1, Azoreductase, Cytochrome b 5 reductase, DHQU, DIA 4, DIA4, Diaphorase (NADH/NADPH) (cytochrome b 5 reductase), Diaphorase (NADH/NADPH), Diaphorase 4, Dioxin inducible 1, DT diaphorase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H dehydrogenase [quinone] 1, NAD(P)H dehydrogenase quinone 1, NAD(P)H menadione oxidoreductase 1 dioxin inducible, NAD(P)H quinone dehydrogenase 1, NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:quinone oxidoreductase 1, NAD(P)H:quinone oxireductase, NMOR 1, NMOR I, NMOR1, NMORI, NQO 1, NQO1, NQO1, Phylloquinone reductase, QR 1, QR1, Quinone reductase 1,
Immunogen: Recombinant protein within human NQO1 1-250 aa.
Conjugate: Unconjugated
Modification: Unmodified