NQO1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90962-50, BS90962-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P15559(Human) Q64669(Mouse) P05982(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IP, , FC
All Applications: WB:1:1,000-1:5,000
FC:1:50-1:100
ICC:1:50-1:200
IP:1:50
Background: NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: NQO1 polyclonal antibody detects endogenous levels of NQO1 protein.
Bioworld Molecular Weight: 31/27 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: Mouse kidney
Lane 3: Raji, Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Alternative Name: DTD, QR1, NQO1, Azoreductase, Cytochrome b 5 reductase, DHQU, DIA 4, DIA4, Diaphorase (NADH/NADPH) (cytochrome b 5 reductase), Diaphorase (NADH/NADPH), Diaphorase 4, Dioxin inducible 1, DT diaphorase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H dehydrogenase [quinone] 1, NAD(P)H dehydrogenase quinone 1, NAD(P)H menadione oxidoreductase 1 dioxin inducible, NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:quinone oxidoreductase 1, NAD(P)H:quinone oxireductase, NMOR 1, NMOR I, NMOR1, NMORI, NQO 1, NQO1, NQO1, Phylloquinone reductase, QR 1, QR1, Quinone reductase 1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified