NR3C1 Polyclonal Antibody-BS90966

NR3C1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90966-50, BS90966-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04150(Human) P06537(Mouse) P06536(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, zebrafish

Applications: WB, FC

All Applications: WB:1:1,000-1:5,000
FC:1:50-1:100

Background: The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NR3C1 polyclonal antibody detects endogenous levels of NR3C1 protein.

Bioworld Molecular Weight: 86 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Glucocorticoid Receptor on different lysates using anti-Glucocorticoid Receptor antibody at 1/1,000 dilution.
Positive control:
Lane 1: zebrafish
Lane 2: A549
Lane 3: HepG2, Flow cytometric analysis of NIH/3T3 cells with Glucocorticoid Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: GR, NR3C1, GCCR, GCR, GCR, GCRST, glucocorticoid nuclear receptor variant 1, Glucocorticoid receptor, GR, GRL, Grl1, nr3c1, Nuclear receptor subfamily 3 group C member 1, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor),

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified