Product Description

NRAS Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66452-50, MB66452-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms . Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimμlates the Raf-MEK-MAPK pathway . GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Applications: WB, IP

Alternate Names: HRAS1, GTPase NRas, Transforming protein N-Ras

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of human Ras

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of NRAS protein.

Molecular Weight: ~ 21 kDa

Pathway: Regμlation of Actin Dynamics, Tumor Angiogenesis, ESC Pluripotency and Differentiation Signaling Pathway, ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, Jak Stat: IL-6 Receptor Signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, NF-kB Signaling, SAPK JNK Signaling Cascades, T cell receptor signaling, TGFβ Smad signaling, Warburg Effect

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P01111

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only