NRDP1 (V276) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: AP6042-50, AP6042-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H4P4
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. Nrdp1, also known as RNF41 (RING finger protein 41), SBBI03 or FLRF, is a 317 amino acid protein that contains one RING-type zinc finger and one SIAH-type zinc finger. Expressed in testis, ovary and prostate, Nrdp1 functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Specifically, Nrdp1 interacts with ErbB-3 and UBPY, thereby targeting them for proteasomal degradation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: NRDP1 (V276) polyclonal antibody detects endogenous levels of NRDP1 protein.
Bioworld Molecular Weight: ~ 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western Blot (WB) analysis of NRDP1 (V276) polyclonal antibody at 1:500 dilution
Lane1:The testis tissue lysate of Rat(40ug)
Lane2:The testis tissue lysate of Mouse(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:SK-OVCAR3 whole cell lysate(40ug)
Alternative Name: E3 ubiquitin-protein ligase NRDP1; RING finger protein 41
Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human NRDP1.
Conjugate: Unconjugated
Modification: Unmodified